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This check can be performed by simply double clicking on the circle to the left of the file name. The Sample Quality Badge can be used to simply access the quality of each of the samples using the metric discussed in Sample Properties. The PhenoGraph algorithm has been implemented… Read more » Sample Quality Check First, it constructs a nearest-neighbor graph to capture the phenotypic relatedness of high-dimensional data points and then it applies the Louvain graph partition algorithm to dissect the nearest-neighbor graph into phenotypically coherent subpopulations. Introduction PhenoGraph is a clustering algorithm that robustly partitions high-parameter single-cell data into phenotypically distinct subpopulations. Iteration by keyword uses a pair of keywords to specify… Read more » Phenograph In this case we can use iterate by keyword. This is due… Read more » Batch Using Iterate by Keywordįor many types of batch reports, simply iterating by the Sample or Panel are the easiest option, but in some cases we need to be more specific about which samples to include in the batch reports. Note: The UMAP plugin for FlowJo requires requires a 64bit OS. (1) The UMAP plugin will run with both FlowJo and SeqGeq bioinformatics data analysis platforms. How-to-UMAP Uniform Manifold Approximation and Projection (UMAP) is a machine learning algorithm used for dimensionality reduction to visualize high parameter datasets in a two dimensional space. The plots are dynamic, can be copied to the clipboard or FlowJo Layout, and allow the user to select populations in one view and highlight the selected population in the other… Read more » UMAP The tool creates an interactive cluster Profile graph, heatmap, and displays the cluster populations on a tSNE/UMAP plot. IntroductionCluster Explorer is a FlowJo plugin.